W. I. PROUT
dually to a trumpet-shaped inner end. It bas a well deHned double outlîoe and a central canal, whicb bas a minute opening at the free end. The latter is 6ij; in diameter, and the trumpet-sbaped end H|JL. Length SÈy.. Beginning just within the orifice is another spicule, which is much longer, 177 1^ and has much the sarae shape, thougb narrower al the point 4 ^. Both sptcules are incurvated foUowing the curvatureoi the lail. and sbow signs of striation, the larger more markedly so.
Pehale worm. — The (emale is loQger and thicker. The total length of tbe head, tail, and intermediate fragments is 40,4<^"i, ur about 16 incbes.
Tbe head is rounded and bluat (lig. 3, A). The tail is hlunt and
sligbtly furved (fig. 3, B), but is nol incurvated like tbe maie tail.
The diameter cluse to the eod is
8 1^4, rapidly increasing to 36(i ut
wliicb it remaitts througbout the
grealer part of the Woim, tapering
more gradually to the head wbere
it measures 4|ji. The body is stria-
ted from end to end, but aot su
markedly as in tbe maie Wonn,
A B and bas a well delioed while chiti-
nous cuticle. Tbe alimentary canal
is straight. but 1 was ucable to
make oui tbe opening of Ibe anus.
At a distance of 435"" (rom the tail, the double utérus, which
bas througbout a well defined external liniog membrane, eau be
observed commenciogin a sac<:ulated extremity. It is bere seen lu
be filled wilh a number of small finely granular cells, somewhat
elongaled and arranged like columnarepithelium, the base against
tbe walls, tbe narrow end towards the lumen of Ihe utérus. A little
furiher on, they increase in size, are pear-shaped, and measure
8iJ.â by 12 !x. At a distance of i\'"° from the lail signs of dilleren-
tationare lirsl to lie seen. Tbey nuw measure from 24 .ui by 12 u- lo
32|j.by 22 [1.. A disliuct cell wrII can now be made oui-
Slil) further up, the proloplasm has shrunk from tbe cell wall aud dilferentiation is more marked. Size 36 u. by 2ft ix. Tbe cells by this time hâve lost their pear shape and are more or less rounded.
OBSERVATIONS OS FILARIA VOLVl'LCS 305
SouR the embryos take on a more deflnite shape, lying curled itp in the cell with c)ear naiDJonic lluid separaling them from a verv distinct liniog mambrane. On staiaing witli logwood the cui-led up embryossbow minute nuclei. Tbe cells vary in six» and average 32!* by 341*.
Following the uteru» up, sonie of Ihe curled embryos aie seeii lo hâve ruptured the cell membi-dne and tu be lying free in Ihe cavity. They gradually uncurl, and Rnally are to be seen packed in rows and interlaceri with one anotber. Development does not proceed at the same rate in bolh branches of the nieras, as in one branch curled embryos can be seen, with tbe olher branch full ot straighl embryos Ivingalongside. The embryos bere measure ISO to 200 |t in lengtb by 4 [t in breadth. Eventually both branches of tbe utérus are seen lo be Tdled wilh siraight embryoii.
The branches ol Ibe ulerus containing embryos can be traced distincly to witliin
l-n-^S of the hiiàd. After (bis t'iK-i-Ï^KmeiitolthebodyotthefemHlr. , , , , sbowiDS the Darrow Inlr^lini' unil twii
owing to Ihe clearness of the ,3^, „,^„, ^„^^^ „^^^, ^^^ryo» «t difspecimen they were more terenl alages ot their dcïelupnunt. diflîcull to follow, and 1 was unable to détermine Ihe vagina or it» opening.
The alimentary opens tcrniinally and at Ihe sides two iinlislimi canals can be niade out.
Embryos. — The free embryos found lying in llie iTSt measurc âoOa in len^lh by iiu loG^iin breadth. The head is roumieil. Thitail is very sharp wilh a laper of aboul 1/5 of llie lengtb of Ibe body. A collection of fine gninutes is seen running along Ihe body.
