PLATE III

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In order to detect the structure of the filament it is necessary, first to discharge it from the licjuid therein contained, tlien to colour it.

If the used solution of chlorinated soda is too strong, thc membrane will be destroyed on several points, the filament will go into pièces and in the préparation will only remains more or less short pièces of it, l3ut which, nevertheless, will allow everybody to get an idea of ils microscopical structure.

FiG. 18, 18' & 18 '. — The main filament is not a mycelial tube, closed up, from distance to distance, by crossiag rectilinear partitions as there are in most of the fungi; it show'S, in the contrary, a spécial structure.

In fact, it is formed by a fasciculus of polyedrical cells, clustering together in an irregular way and constituting a real cellular tissue, so that ils terni of » a filament » is a wrong one.

The cells are fiUing up entirely the whole thickness of the filament, leaving no free space therein. They are so sniall that at the first look they are taken for granulations of the membrane; somc of them are of a greater size and constitute the vacuoles. In fact, they are containing the niany oildrops which the filament shows ou the whole of its length.

FiG. 19. — The structure of the filament is not a uniform one in its whole length; tlie nuniber and the position of the cells varying according to the point where they are situated.

FiG. 19\ — Sometimes, between two points wiiere the cellular tissue is well developped, the filament consists only of a single range of cells.

FiG. 19 & 19 '. — The tissue is slowly getting simplified towards the ends of the lilament, where the latter shows thus the real thready structure.

FiG. 20. — Structure of filament on the spots where there is a branching off.

FiG. 21. — Tliei'c may 1)C sccu stilj in llic pruparalions <i gi'Cul deal of \evy siiiall thready pioces, somo of lliciii conta iiiing twi) or thi'oc cclls. Biffgcr ones, of a round or longtliciicd sliapc, conlain onc oi' more vacuoles, wliicli werc bclievcd to be (llilaiiiy-

dospoi'cs.

FiG. 22. — Certain spherical strunias on small branches arc tlic pscudoconidea which secuied to J)e siniilar to those of thc Mortierella. Perhaps ouglit we to considcr theni as small bnll)s and as playing a part in thc rcprodncing processus?

FiG. 22\ — Thèse strnmas are sometimes prolonged by a filament.

When treating thc white Mycélium of truftlc by means of a strongly diluted solution of hyperchlorite and when Icaving it long enough exposed to its action, one succeeds in perceiving its structure without destroying the gênerai sight of the fungus.

Wc may sec then that the main filament is formed by sevcral sections, which, put together, constitutc its whole length. The number. of cells comprised within the thickness of the filament, varies according to said thickness and the tissue, first well developped goes down, towards the ends, to a singular range of cells.

FiG. 23. — \t the ends of tlic filament the latler is sometimes composed of a particular kind of tissue; said tissue, a very rudimentary one, being only constituted by groups of few cells, separate from eachothcr and situate, at a regular distance froni eachother, in the mycelious tube and a strange feature is that said tube is not quite closcd by said tissue on the points where it developped itself.

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